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ActoFactor™ Recombinant Human Chemokine (C-X-C motif) ligand 14
- Specification
Cat.No.
CSC-CTK0493
Description
Breast and Kidney-expressed chemokine (BRAK) is a CXC chemokine expressed in normal tissue in the absence of inflammatory stimuli, and infrequently expressed in cancer cell lines. BRAK is known to be a highly selective monocyte chemoattractant. However, main function and receptor selectivity is unknown at this time. BRAK contains the four highly conserved cysteine residues present in CXC chemokines. The sequence of the mature protein consists of 87 amino acid residues, and is approximately 30% homologous to the sequences of MIP-2 alpha and beta. Recombinant human BRAK is a 9.4 kDa protein containing 77 amino acid residues.
Species
Human
Product Overview
Human CXCL14 expressed in E.coli
Molecular Mass
9.4 kDa
Size
CAT# CSC-CTK0493-20 (20 μg); CAT# CSC-CTK0493-100 (100 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by ist ability to chemoattract activated monocytes using a concentration of 1-10 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered (0.2 micron filter) solution with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted BRAK should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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