ActoFactor™ Recombinant Human Chemokine (C-X-C motif) ligand 13

Specification

Cat.No.

CSC-CTK0431

Description

BCA-1/BLC, a CXC chemokine, is expressed in the liver, spleen, lymph nodes, appendix and stomach. It exerts its activities through its only receptor CXCR5. BCA-1/BLC is a potent chemoattractant for B lymphocytes and induces weak chemotactic response in T cells and macrophages. It manifests no activity on neutrophils and monocytes. Recombinant human BCA- 1 is a 10.1 kDa protein containing 85 amino acid residues including the four highly conserved cysteine residues present in CXC chemokines.

Species

Human

Product Overview

Human CXCL13 expressed in E.coli

Molecular Mass

10.1 kDa

Size

CAT# CSC-CTK0431-20 (20 μg); CAT# CSC-CTK0431-100 (100 μg)

Expression System

E.coli

Purity

Greater than 97% as determined by SDS-PAGE and HPLC analysis.

Activity

Determined by its ability to chemoattract human B cells using a concentration range of 1.0-10.0 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered solution with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted BCA-1 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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