ActoFactor™ Recombinant Human Chemokine (C-X-C motif) ligand 12 (beta)

Specification

Cat.No.

CSC-CTK0535

Description

Recombinant Human Stromal Cell-Derived Factor-1beta (rHu SDF-1 beta) is a recently discovered protein belonging to the a-chemokine (C-X-C) family of cytokines. Human SDF-1 beta is an 8.5 kDa protein containing 72 amino acid residues.

Species

Human

Product Overview

Human CXCL12 expressed in E.coli

Molecular Mass

8.5 kDa

Size

CAT# CSC-CTK0535-10 (10 μg); CAT# CSC-CTK0535-50 (50 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.

Activity

The activity of rHu SDF-1 beta was determined by its ability to chemoattract human peripheral T- cells activated with PHA and IL-2. This effect was reached using a concentration range of 20.0- 80.0 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a concentrated (1 mg/ml), sterile protein solution with no additives.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized SDF-1 beta, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted human SDF-1 beta should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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