ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 3-like 1

Specification

Cat.No.

CSC-CTK0460

Description

LD78-beta is a CC chemokine that is closely related to MIP-1 alpha. It signals through the CCR5 receptor and the beta-chemokine receptor, D6. LD78-beta has been shown to exhibit potent activity in HIV suppression assays. Recombinant human LD78-beta is a 7.7 kDa protein containing 70 amino acid residues, including the four conserved cysteine residues present in CC chemokines.

Species

Human

Product Overview

Human CCL3L1 expressed in E.coli

Molecular Mass

7.7 kDa

Size

CAT# CSC-CTK0460-20 (20 μg); CAT# CSC-CTK0460-100 (100 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

Determined by its ability to chemoattract human monocytes using a concentration range of 1.0- 10.0 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized (freeze-dried), sterile powder. Lyophilized with no additives.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu LD78-beta should be used immediately or stored long- term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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