ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 28

Specification

Cat.No.

CSC-CTK0577

Description

MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.

Species

Human

Product Overview

Human CCL28 expressed in E.coli

Molecular Mass

12.3 kDa

Size

CAT# CSC-CTK0577-20 (20 μg); CAT# CSC-CTK0577-100 (100 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

Determined by its ability to chemoattract human lymphocytes using a concentration range of 1.0-10.0 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered solution in 10 mM acetic acid.

Reconstitution

Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MEC should be used immediately or stored in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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