ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 27

Specification

Cat.No.

CSC-CTK0494

Description

Recombinant Human CTACK (rHu CTACK) is a keratinocyte derived CC chemokine, which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selevtively attracts CLA+ T-cells and directs them into the skin. Recombinant Human CTACK is a 9.8 kDa protein containing 86 amino acid residues.

Species

Human

Product Overview

Human CCL27 expressed in E.coli

Molecular Mass

9.8 kDa

Size

CAT# CSC-CTK0494-20 (20 μg); CAT# CSC-CTK0494-100 (100 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

The activity of rHu CTACK was determined by its ability to chemoattract CXCR3 transfected cells. This effect was reached using a concentration range of 10-100 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized protein with no additives.

Reconstitution

Please centrifuge product before opening vial. The lyophilized protein should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized rHu CTACK, though stable at room temperature, is best stored desiccated at - 20°C. Reconstituted human CTACK should be stored in working aliquots at -20°C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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