ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 23

Specification

Cat.No.

CSC-CTK0462

Description

MIP-3 is a CC chemokine that signals through the CCR1 receptor. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but does not chemoattract activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3 is an 11.3 kDa protein containing 99 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Species

Human

Product Overview

Human CCL23 expressed in E.coli

Molecular Mass

11.3 kDa

Size

CAT# CSC-CTK0462-20 (20 μg); CAT# CSC-CTK0462-100 (100 μg)

Expression System

E.coli

Purity

Greater than 99% as determined by SDS-PAGE and HPLC analysis.

Activity

Determined by its ability to chemoattract human T cell population using a concentration of 10-50 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered solution with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted MIP-3 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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