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ActoFactor™ Recombinant Human Chemokine (C-C motif) ligand 2
- Specification
Cat.No.
CSC-CTK0367
Description
The MCP proteins belong to the CC chemokine family, and signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated T cells, basophils, NK cells, and immature dendritic cells. Recombinant human MCP-1 is an 8.6 kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
Species
Human
Product Overview
Human CCL2 expressed in E.coli
Molecular Mass
8.6 kDa
Size
CAT# CSC-CTK0367-20 (20 μg); CAT# CSC-CTK0367-100 (100 μg)
Expression System
E.coli
Purity
Greater than 99% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to chemoattract human monocytes using a concentration range of 5.0- 20.0 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized protein with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu MCAF should be used immediately or stored long-term in undiluted working aliquots at -20°C. Please avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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