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ActoFactor™ Recombinant Human CD4 molecule
- Specification
Cat.No.
CSC-CTK0109
Description
CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MHC (Major Histocompatibility Complex) Class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as HIV receptors, binding directly to the envelope protein gp120 on HIV. Thymocytes which are CD4 (+) and CD8 ( - ) produce factors such as IL-4, IL-5, IL-10 and IFN-gamma. Recombinant Human sCD4 produced in Baculovirus is a single, glycosylated polypeptide chain of 365 amino acids and a non-glycosylated molecular mass of 45,000 Dalton. As a result of glycosylation, the sCD4 migrates as a 46,000 Dalton protein on standard SDS-PAGE.
Species
Human
Product Overview
Human CD4 expressed in Baculovirus
Molecular Mass
45 kDa
Size
CAT# CSC-CTK0109-10 (10 μg); CAT# CSC-CTK0109-50 (50 μg)
Expression System
Baculovirus
Purity
Greater than 90% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
Recombinant human sCD4 is immune-reactive using monoclonal anti-CD4 antibodies in an gpl2O capture ELISA assay.
Endotoxin Level
Less than 1 EU/μg.
Formulation
The sterile-filtered, colourless sCD4 solution contains 10 mM sodium phosphate buffer (pH 7.0) and 300 mM sodium chloride.
Reconstitution
Please centrifuge product briefly before opening vial. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if the entire vial will be used within 1 week. Store, frozen at -20°C for longer periods of time. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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