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ActoFactor™ Recombinant Human Bone morphogenetic protein 7
- Specification
Cat.No.
CSC-CTK0533
Description
The bone morphogenetic proteins are a family of secreted signaling molecules that can induce ectopic bone growth. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (Chen et al., 2004, Kishigami and Mishina 2005). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (Sampath et al., 1992, Kazama et al., 2008), inhibits the branching of prostate epithelium (Grishina et al., 2005), and antagonizes epithelialmesenchymal transition (EMT) (Zeisberg et al., 2003, Yu et al., 2009).In pathological conditions, BMP-7 inhibits tumor growth and metastasis (Buijs et al., 2007), ameliorates fibrotic damage in nephritis (Zeisberg et al., 2003), and promotes neuroregeneration following brain ischemia (Chou et al., 2006). Recombinant BMP-7 has been produced by transient expression of BMP-7 in non-transgenic plant. It consists of a 16.5 kDa single chain with 144 amino residues and contains a 6-His-tag at the N-terminal end. BMP-7 has been purified by sequential chromatography (FPLC) and does not contain animal–derived components or impurities.
Species
Human
Product Overview
Human BMP7 expressed in Nicotiana benthamiana
Molecular Mass
16.5 kDa
Size
CAT# CSC-CTK0533-10 (10 μg); CAT# CSC-CTK0533-50 (50 μg)
Expression System
Nicotiana benthamiana
Purity
Greater than 97% as determined by SDS-PAGE analysis.
Activity
The biological activity of BMP-7 is measured by ist ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 was found to be ≤40 ng/ml.
Endotoxin Level
Less than 0.04 EU/μg.
Formulation
Lyophilized from a 0.05 M Tris-HCl buffer (pH 7.4).
Reconstitution
Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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