ActoFactor™ Recombinant Human Interleukin 4 (E. coli)

Specification

Cat.No.

CSC-CTK0223

Description

Human Interleukin-4 (rHu IL-4) is a 15 kDa protein containing 130 amino acid residues. rHu IL-4 is a potent lymphoid cell growth factor, which stimulates the growth and survivability of certain B-cells and T-Cells.

Species

Human

Product Overview

Human IL4 expressed in E.coli

Molecular Mass

15 kDa

Size

CAT# CSC-CTK0223-10 (10 μg); CAT# CSC-CTK0223-50 (50 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

The specific biological activity was determined after lyophilisation in direct comparison with the WHO standard (WHO international standard 88/656, NIBSC) by a standard cell proliferation assay using a human factor-dependent cell line TF-1. The ED50 for this effect is <0.2 ng/ml, corresponding to a specific activity of 5.0 x 10^6 units/mg.

Endotoxin Level

N/A

Formulation

Lyophilized from sterile-filtered protein solution (1 mg/ml) in water with no additives.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze/thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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