8-MG-BA

Cat.No.: CSC-C0467

Species: Human

Source: frontal brain lobe

Morphology: epithelial-like, adherent cells growing as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0467

Description

Established from the frontal brain lobe of a 54-year-old woman with glioblastoma multiforme in 1989 (pre-treatment)

Species

Human

Source

frontal brain lobe

Recommended Medium

80-85% EMEM + 15-20% h.i. FBS

Culture Properties

monolayer

Morphology

epithelial-like, adherent cells growing as monolayer

Karyotype

Human flat-moded hyperdiploid karyotype with 15% polyploidy - 52(47-52)<2n>X, -X, +1, +1, +3, +5, +6, +7, +8, -19, +2-4mar, add(1)(p34-36;hsr), add(1)(p34-36), der(1)t(1;17)(q11-12;q12-21), der(3;5)(q10;q10), i(5p), del(6)(q24-25), del(7)(p14), i(7p), i(8

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin (+), cytokeratin-7 -, cytokeratin-8 (+), cytokeratin-17 -, cytokeratin-18 -, cyto

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What conditions require contacting staff upon receipt of cells?

If you find that the dry ice has completely evaporated, the cap of the lyophilisation tube has fallen off or is broken, or the cells are contaminated, please contact the staff immediately.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Multiple purchases

I bought Creative Bioarray's cellular products on the recommendation of a friend, and I have returned many times since then.

13 Mar 2022

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For research use only. Not for any other purpose.