1321N1

Cat.No.: CSC-C9421J

Species: Human

Specification
Q & A
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Cat.No.

CSC-C9421J

Description

1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten (Pontén, J., Macintyre, E. H. (1968) PMID: 4313504).1321N1 has been shown to have very similar STR profile data to U-118 MG.

Species

Human

Tissue of Origin

brain

Recommended Medium

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Storage

Liquid Nitrogen (-180 °C).

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What happens to the cells after mycobacterial contamination occurs during cell culture?

When observed under an ordinary inverted microscope, flocculent interlaced hyphae or clusters are visible growing between the cells; sometimes the fungus is not directly distributed in the cell adlayers and requires careful observation by adjusting the microscope to look for hyphae or clusters.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Helpful

With newly resuscitated cells, I make sure to freeze a tube in the first generation, which is very helpful for my experiments.

24 Apr 2021

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For research use only. Not for any other purpose.