Multi-Differentiation of Peripheral Blood Mononuclear Cells

Peripheral blood mononuclear cells (PBMCs) are a diverse group of immune cells that play a crucial role in the body's immune response. They include lymphocytes (B cells, T cells, and natural killer cells), monocytes, and dendritic cells. PBMCs have been reported to contain a multitude of distinct multipotent progenitor cell populations and possess the potential to differentiate into blood cells, endothelial cells, hepatocytes, cardiomyogenic cells, smooth muscle cells, osteoblasts, osteoclasts, epithelial cells, neural cells, or myofibroblasts under appropriate conditions.

Multi-Differentiation of PBMCs

• Differentiation of PBMCs into blood cells
  PBMCs can differentiate into various types of blood cells, including red blood cells (erythrocytes), white blood cells (leukocytes), and platelets. Human PBMCs can also produce other hematopoietic colonies, including colony-forming unit-granulocyte-erythroid monocyte-macrophage-megakaryocyte, colony-forming unit monocyte-macrophage, blast-forming unit-erythroid and colony-forming unit-erythroid precursors.
• Differentiation of PBMCs into endothelial cells
  Endothelial cells form the inner lining of blood vessels and play a critical role in angiogenesis and vascular repair. PBMCs can be differentiated into endothelial cells through a combination of growth factors and culture conditions. This process mimics the natural development of endothelial cells from precursor cells during embryogenesis.
• Differentiation of PBMCs into hepatocytes
  Human PBMCs have the potential to differentiate into hepatocyte-like cells in vitro in the presence of hepatocyte growth factor or fibroblast growth factor (FGF)-4. During induction culture, cells gradually rounded but did not assume the polygonal shape of mature hepatocytes, which might suggest additional signals are required for full differentiation.
• Differentiation of PBMCs into muscle cells
  Growth factors such as transforming growth factor and platelet-derived growth factor-BB participate in smooth muscle cell (SMC) differentiation. Human PBMCs grown in platelet-derived growth factor BB-enriched medium differentiated into smooth muscle outgrowth cells that were positive for SMC-specific actin (SMA), myosin heavy chain, and calponin at both the mRNA and protein levels.
• Differentiation of PBMCs into neural cells
  The nervous system is composed of neurons and glial cells, which coordinate and regulate various physiological processes. PBMCs can differentiate into neural cells, including neurons and astrocytes, through the activation of specific signaling pathways and culture conditions.

Methods for the Isolation and Purification of PBMCs

Fig. 1 Schematic diagram for separation of PBMC.

• The isolation and purification of PBMCs from peripheral blood are crucial steps for downstream applications. Several methods are commonly used for PBMC isolation, including density gradient centrifugation, magnetic bead separation, and microfluidic techniques.
• In density gradient centrifugation, blood is layered on top of a density gradient medium, and centrifugation separates the PBMCs from other blood components based on their density. Magnetic bead separation utilizes specific antibody-coated magnetic beads to selectively bind and isolate PBMCs from other cell types. Microfluidic techniques offer a more automated and high-throughput approach for PBMC isolation.

Creative Bioarray Relevant Recommendations

Creative Bioarray provides a high-quality and comprehensive range of PBMCs, to accelerate our customers' scientific research. Specifically, our products include but are not limited to Human Normal Peripheral Blood Mononuclear Cells (PBMCs), Human MULTIPLE MYELOMA, Peripheral Blood Mononuclear Cells, and Human Peripheral Blood Mononuclear Cells (HPBMC).

For research use only. Not for any other purpose.