SuperQuick® UGT Activity Assay Screening Kit

DMK-YS011

UGTs are expressed primarily in the liver, intestine, and kidney and catalyze the incorporation of the glucuronide moiety of the nucleotide urea-5'-diphosphate-α-D-glucuronide (UDPGA) into the nucleophilic hydroxyl, sulfhydryl or amino groups of pharmacologically active drugs, phase I drug metabolites, and specific endogenous substrates such as steroid hormones, bile acids, and bilirubin.UGTs also promote first-pass effect pre-drug metabolism, which significantly reduces the oral bioavailability of some drugs. Creative Bioarray's UGT Activity Assay Screening Kit allows quick examination of native/recombinant UGT activity in biological samples such as liver microsomes and can also provide an evaluation of the effect of novel compounds on UGT activity.

To rapidly assess native or recombinant UGT activity in fractions prepared from tissues and cells; to screen for drug and new ligand interactions with native or recombinant UGT enzymes.

Simple and ready to use;
Rapid and high-throughput;
Hight sensitive;
Non-radioactive.

Liver microsomes and liver S9 fractions;
Tissue and cultured cell lysates, primary hepatocytes;
Heterologous expression of recombinant UGT preparations.

UGT Assay Buffer;
UGT Inhibitor (Diclofenac);
UDPGA Stock(50X);
Alamethicin;
UGT Substrate(250X);
UGT Positive Control.

100 assys

Strore at -20℃.

Gel Pack

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.