SuperQuick® Cytochrome P450 3A4 (CYP3A4) Activity Assay Kit

DMK-YS001

CYP3A4 is abundantly expressed in the liver and intestine, where it catalyzes the oxidation of a variety of structurally different ligands. Of all small molecule drugs commonly used in humans, more than half are metabolized by CYP3A4, and inhibition of CYP3A4-mediated metabolism is a common cause of drug/drug and drug/food interactions and toxicity.In addition, for the pharmacological activity of a drug to be metabolized from the prodrug form, inhibition of CYP3A4 can lead to decreased drug efficacy.Creative Bioarray's CYP3A4 Activity Assay Kit allows rapid measurement of native or recombinant CYP3A4 activity in biological samples, such as liver microsomes.

Rapid assessment of native/recombinant CYP3A4 activity in fractions prepared from tissues and cells.
Screening compounds for interaction with native/recombinant CYP3A4.

Simple and ready to use;
With little interference by autofluorescence.
Rapid and hight sensitive.

Liver microsomes and liver S9 fractions;
Tissue and cultured cell lysates, primary hepatocytes;
Heterologous expression of recombinant CYP3A4 preparations.

CYP3A4 Assay Buffer
Resorufin Standard (5 mM in DMSO);
CYP3A4 Inhibitor (Ketoconazole);
NADPH Generating System(100X);
β-NADP+Stock (100X);
CYP3A4 Substrate;
Recombinant Human CYP3A5.

200 assys

Strore at -20℃.

Gel Pack

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