Myelin Basic Protein (MBP)-Induced Experimental Autoimmune Encephalomyelitis (EAE) Model

Creative Bioarray offers a myelin basic protein (MBP)-induced Experimental Autoimmune Encephalomyelitis (EAE) model to support and advance multiple sclerosis (MS) research. Our EAE model is designed to mimic the key aspects of MS, providing a reliable platform for studying disease mechanisms and evaluating potential therapeutic agents. By utilizing our services, researchers can obtain consistent and reproducible results, ultimately accelerating the development and testing of new MS treatments. With us, you can enhance the efficiency and effectiveness of your drug development process.

Overview of EAE Model

EAE is an antigen-driven autoimmune model of MS in which immunization against myelin antigens elicits strong T-cell responses that initiate its pathology with central nervous system (CNS) myelin destruction. EAE is induced by immunization with CNS tissue or myelin peptides. Different immunization protocols result in varying disease characteristics.

Introduction of MBP

MBP constitutes 30%-40% of the myelin proteins within the CNS and 5%-15% of those in peripheral nerves. It was among the earliest proteins successfully purified from spinal cord homogenates and demonstrated to provoke EAE. MBP-induced EAE manifests as a severe form of encephalomyelitis, characterized by an acute, monophasic disease course. This model closely simulates the pathological features of MS, providing a critical tool for studying the disease's mechanisms and testing potential therapeutic interventions.

Our Myelin Basic Protein (MBP)-Induced Experimental Autoimmune Encephalomyelitis (EAE) Model

• Features of MBP-Induced EAE Model in Creative Bioarray

• Model Characteristics: This acute, self-limiting model exhibits minimal to no demyelination and is characterized primarily by T cell infiltration in the central nervous system (CNS).
• Disease Onset and Duration: Disease onset occurs approximately 10-13 days post-immunization, with the experiment typically concluding around day 20. This makes it the shortest available EAE model from Creative Bioarray.

• Available Animal

Lewis rat (female)

• Modeling Method

For induction of EAE, the Lewis rats are immunized via injection of MBP into their hind footpads.

• Endpoints

• Body weight
• Clinical score
• Histology analysis
• Cytokine analysis
• qPCR or Western blot
• Other customized endpoints

Example Data

Fig. 1 PZH ameliorated CNS inflammation in EAE rats. Rats were sacrificed at day 31, and the brain, brainstem, and spinal cord were harvested. Inflammation of the brain, brainstem, and spinal cord was analyzed by H&E staining. (Qiu et al. 2018)

In addition, we also provide other EAE models that maybe you are interested in:

• MOG_35-55-Induced Experimental Autoimmune Encephalomyelitis (EAE) Model
• Proteolipid Protein (PLP)-Induced Experimental Autoimmune Encephalomyelitis (EAE) Model

Quotation and Ordering

Creative Bioarray is a distinguished Contract Research Organization (CRO) that focuses on preclinical studies related to inflammation and autoimmune diseases. We are dedicated to the principle that advancing critical research relies on offering our clients exceptional animal model solutions. Our goal is to be a pivotal partner in the preclinical research phase, ensuring that our clients have the necessary tools and support to make groundbreaking discoveries in the fields of inflammation and autoimmunity. If you are interested in our services, please feel free to contact us at any time or submit an inquiry directly to us.

Reference

1. Qiu, X., et al. Therapeutic potential of Pien Tze Huang on experimental autoimmune encephalomyelitis rat. Journal of immunology research, 2018, 2018(1): 2952471.

For research use only. Not for any other purpose.