High-Fat Diet-Induced Atherosclerosis Model

Creative Bioarray offers a well-established atherosclerosis model induced by a high-fat diet, available to customers worldwide. With our state-of-the-art technology and experienced experts, we ensure our global clients receive the most reliable service at the most competitive price. Our team of professional scientists is dedicated to helping clients and expediting their preclinical research programs.

Atherosclerosis is the pathology of numerous cardiovascular diseases (CVDs), posing a persistent threat despite the widespread adoption of lipid-lowering medications, interventional therapies, and other conventional treatments. It remains the world's leading cause of mortality, exacerbated by chronic exposure to cardiovascular risk factors including hyperlipidemia, hypertension, smoking, and diabetes. To advance our understanding and treatment of this condition, high-fat diet-induced atherosclerosis has become indispensable. This model offers a critical research platform, enabling scientists to rigorously assess the efficacy of novel drug candidates in the battle against atherosclerosis and its associated CVDs.

Our High-Fat Diet-Induced Atherosclerosis Model

Available Animal

Rat
Mouse

Modeling Method

1) Apoe^-/- Mouse: Apoe^-/- mice are fed with high-fat diet for 12-16 weeks to induce atherosclerosis model.

Schematic illustration depicting the methodology for inducing atherosclerosis in Apoe-/- mice, highlighting the key steps involved in the model creation process. Fig. 1 Schematic diagram of the method of atherosclerosis modeling in Apoe^-/- mice.

2) Rat: Atherosclerosis is induced in rats by feeding them a high-fat diet for 13 weeks, with intraperitoneal injection of vitamin D3 administered at intervals of 1 week, 3 weeks, 6 weeks, and 9 weeks.

Schematic illustration depicting the methodology for inducing atherosclerosis in rats, highlighting the key steps involved in the model creation process. Fig. 2 Schematic diagram of the method of atherosclerosis modeling in rat.

Endpoints

Atherosclerotic plaques observation
Histology analysis: H&E staining, Oil Red O staining, Masson staining, Sirius Red staining, IHC
Serum analysis: TG, TC, HDL-C, LDL-C
Body weight
qPCR or Western blot
Other customized endpoints: available upon request

Example Data

Schematic diagram of the effects of berberine on atherosclerosis induced by a high-fat diet in mice including Oil Red O staining of the aorta, H&E-stained aortic root sections, and quantitative assessments of plaque and lesion sizes. Fig. 3 Berberine attenuates HFD-induced atherosclerosis. (A) Representative images of en face Oil Red O staining of aorta. (B) H&E staining of aortic root. (C and D) Quantitative analysis of plaque area in aorta and lesion size in the aortic root. (Wu et al. 2020)

Schematic diagram of berberine's impact on serum lipid profiles in high-fat diet-fed ApoE-/- mice, showing levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol. Fig. 4 Berberine ameliorates serum lipid levels in HFD-fed ApoE–/– mice. (A) Total cholesterol, (B) triglyceride, (C) low-density lipoprotein cholesterol, (D) high density lipoprotein cholesterol. (Wu et al. 2020)

Quotation and Ordering

Creative Bioarray is dedicated to creating a wide range of disease models to support and enhance preclinical research programs. If you are interested in our services, please feel free to contact us at any time or submit an inquiry to us directly.

Reference

Wu, M., et al. Effect of berberine on atherosclerosis and gut microbiota modulation and their correlation in high-fat diet-fed ApoE−/− mice. Frontiers in pharmacology, 2020, 11: 223.

For research use only. Not for any other purpose.