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QualiNuclei® Plasma/Serum RNA Extraction Kit
- Specification
Cat.No.
CNEK-R1812H
Description
QualiNuclei® Plasma/Serum RNA Extraction Kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two-column method. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Applications
PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Size
10 preps/20 preps/50 preps
Storage & Stability
Reagents should remain stable for up to 2 year in their unopened containers.
Storage
All buffers should be kept tightly sealed and stored at room temperature.
Warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Shipping
Room temperature or blue ice
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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