QualiNuclei® Animal Tissue RNA Extraction Kit

Specification

Cat.No.

CNEK-R1805H

Description

QualiNuclei® Animal Tissue RNA Extraction Kit is designed for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine and more. Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins and connective tissues. This kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. If the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

Applications

qRT-PCR, RT-PCR, microarrays, NGS and more.

Size

50 preps

Storage & Stability

Reagents should remain stable for at least 1 year in their unopened containers

Storage

All solutions should be kept tightly sealed and stored at room temperature.
The DNAse I should be stored at -20°C upon arrival.
The Proteinase K should be stored at -20°C upon arrival and after reconstitution.

Shipping

Room temperature or blue ice

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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