- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
Peripheral Blood Mononuclear Cells
Peripheral blood mononuclear cells (PBMCs) are cells with a single, round nucleus and are collected from the peripheral or circulating blood by density centrifugation with Ficoll, a polysaccharide. PBMCs include dendritic cells, monocytes, and lymphocytes (T, B, and NK cells). T cells are CD3+ and further classified into helper T cells (TH) that express CD4 and cytotoxic T cells (TC) that express CD8. B cells are characterized by CD19 expression and respond to pathogens by producing a large number of antigen-specific antibodies to neutralize foreign objects. NK cells are CD56+CD3-, which originate from the same progenitor cells as T and B cells, but lack the antigen receptor. Finally, monocytes are the largest type of PBMCs and express CD14. They circulate in the bloodstream for 1-3 days and then usually move into tissues throughout the body, where they can differentiate into macrophages and myeloid-lineage dendritic cells.
| Cell Type | Percentage in PBMCs | Markers | Function |
| CD4+ T cells | 25-60% | CD3+CD4+ | Coordinate adaptive immunity through activation and regulation of other immune cells |
| CD8+ T cells | 5-30% | CD3+CD8+ | Destroy cancer cells or cells that are infected or damaged |
| B cells | 5-10% | CD19+ | Secrete antibodies as part of humoral immune response |
| NK cells | 10-30% | CD56+CD3- | Trigger lysis or apoptosis of infected cells |
| Monocytes | 5-10% | CD14+ | Take up foreign objects via phagocytosis, perform antigen presentation, and produce cytokines |
| Dendritic cells | 1-2% | Process and present antigen material to T cells |
PBMCs are a powerful tool for research and clinical studies related to human health and disease. By efficient and successful processing and analysis of PBMCs, researchers and clinicians can evaluate immune responses, gain a deeper understanding of the immune system, and apply their findings to the treatment of human diseases. PBMC are now readily available from many commercial sources, with samples isolated from both healthy and diseased individuals.
Description: Mononuclear cells (Mononuclear Cells) are small aliquots from an apheresis leuko...
Description: NPB-Macrophages are derived from negatively-selected peripheral blood monocytes and...
Description: RBC depleted leukocytes are collected from peripheral blood by sedimentation of red...
Description: NPB-Monocyte Derived Myeloid Dendritic Cells (MoDC) are derived from highly purified...
Description: NPB-CD34+ stem/progenitor cells (NPB-CD34+) are positively isolated from mononuclear...
Description: NPB-BDCA4+ Lymphoid/Plasmacytoid Dendritic cells have been positively isolated from...
Description: NPB-Basophils are isolated from peripheral blood Mononuclear Cells using an indirect...
Description: Multiple Myeloma-PB-Plasma is obtained by centrifugation of Multiple Myeloma peripheral...
Description: Multiple Myeloma-PB-Plasma is obtained by centrifugation of Multiple Myeloma peripheral...
Description: Multiple Myeloma-PB-Mononuclear Cells are isolated from Multiple Myeloma-peripheral...
Description: Multiple Myeloma-PB-Mononuclear Cells are isolated from Multiple Myeloma-peripheral...
Description: Multiple Sclerosis (MS) peripheral blood plasma is obtained by centrifugation of...
Description: Peripheral blood mononuclear cells are isolated from Multiple Sclerosis (MS) peripheral...
Description: MPB-CD34 Depleted Mononuclear Cells are isolated using immunomagnetic CD34 MicroBead...
Description: These mononuclear cells (Mononuclear Cells) are collected using the COBE Spectra...
Description: MPB-CD56+ Natural Killer (NK) Cells are positively isolated from mononuclear cells...
Description: MPB-CD34+ Stem/Progenitor cells are positively selected using a direct immunomagnetic...
Description: MPB-CD14+ Monocytes are positively isolated from MPB-Mononuclear Cells using a direct...
