OCI-AML-5

An Image-Based Flow Cytometric Approach to Assess N: C Ratio of OCI-AML-5 Cell Lines

The nucleus-to-cytoplasm ratio (N: C) can be used as one metric in histology for grading certain types of tumor malignancy. OCI-AML-5, a blood cancer cell line, was interrogated by an image-based flow cytometer (IFC). Initially, 43,237 OCI-AML-5 cells were imaged using the IFC within minutes. In the IFC gating process, out-of-focus cells, cell images containing alignment beads, and multiple or fragmented cells were excluded. Objects analyzed in the IDEAS software indicated a larger nucleus mask than a cytoplasm mask. From this post-processing, 5284 OCI-AML-5 cells were able to be analyzed (Fig. 1).

Table 1 shows that the diameter of OCI-AML-5 cells (12.3 ± 1.2 μm) is consistent with previously published values obtained using the Coulter Counter. The nuclear diameter of these cells follows a similar trend, with OCI-AML-5 cells measuring 9.0 ± 1.1 μm. Based on these measurements, the N: C ratio was calculated for each cell line. The N: C ratio for OCI-AML-5 cells was 0.73 ± 0.07.

Fig. 1 IFC analysis workflow and representative cells. (Sebastian JA, et al., 2021)

Table 1. A summary of IFC measurements and corresponding standard deviations for the cell and nucleus diameter as well as the N: C ratio for the OCI-AML-5 cell line. (Sebastian JA, et al., 2021)