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Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2)

Cat.No.: CSC-I9247L

Source: Brain

Morphology: Composed of round and broad flat polygonal-like appearance

Culture Properties: Adherent

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Cat.No.
CSC-I9247L
Description
Immortalized Rat Suprachiasmatic Nucleus Cells (SCN2.2) is pluripotent and has the appearance of both neuronal and glial morphologies in culture. The cells are able to differentiate into different phenotypes, where neuronal markers are expressed (NSE, PGP, and MAP-2), but glial markers are absent. It is a valuable tool for studies relating to the regulation of the central mammalian pacemaker function, and the development of the suprachiasmatic nucleus cell types.
Source
Brain
Culture Properties
Adherent
Morphology
Composed of round and broad flat polygonal-like appearance
Immortalization Method
Serial passaging and transduction with retrovirus carrying adenovirus 12S E1A gene
Markers
NSE, PGP, and MAP-2
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Immunofluorescent staining and western blot analysis for E1A;
2) Immunocytochemical screening with cell type-specific antigens to distinguish phenotypes.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.