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Immortalized Mouse Microglia (SIM-A9)
Cat.No.: CSC-I9208L
Species: Mus musculus
Source: Postnatal mouse cerebral cortices
Culture Properties: Adherent
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Note: Never can cells be kept at -20 °C.
2) ELISA was used to determine the TNFα secretion after inflammatory stimulation;
3) Western blot and immunocytochemistry were used to confirm the expression of microglial specific markers such as CD68 and Iba1 and M1/M2 phenotype-associated protein expression (COX-2/iNOS and Arg-1) after stimulation;
4) Aβ1-42orE. coli-derived bioparticles uptake was used to determine phagocytic activity of SIM-A9 cells.
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Description: Follicle stimulating hormone (FSH) receptor, a receptor uniquely expressed in ovarian granulosa and testicular sertoli cells, play an important role in the regulation of oogenesis, spermatogenesis and steroid hormone production. Unfortunately FSH receptor expression is often lost within 1-2 days ofin vitroculture of primary cells. The Immortalized Rat Steroidogenic Granulosa Cells stably expressing FSH Receptor is responsive to FSH stimulation and produces progesterone as a result at levels comparable to primary cells. This cell line is useful in studying FSH-FSH receptor interactions, as well as how FSH receptor regulates the development and maturation of ovarian follicle. Together with theImmortalized Rat Steroidogenic Granulosa Cells expressing LH Receptor, researchers can compare the LH and FSH signaling in homologues cell systems.
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