Description
Mouse lung fibroblasts were isolated and transfected with SV40LT expressing lentiviral particles. These primary cells go into senescence after the 2nd passage while the Immortalized rat brain microvascular endothelial cells go beyond 30 passages.
Recommended Medium
SuperCult® Immortalized Mouse Lung Fibroblast Medium (Cat No.: CM-I2056Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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What are Immortalized Mouse Lung Fibroblasts?
Immortalized Mouse Lung Fibroblasts are lung fibroblast cells derived from mice that have been genetically modified to go beyond 20 passages in vitro. They retain critical features of primary lung fibroblasts, making them a valuable model for respiratory and fibrotic research.