Description
Nonciliated bronchiolar (Clara) cells, alveolar type I and type II cells are the predominant cell types in the respiratory epithelium. Clara cells and alveolar type II cells produce pulmonary surfactant proteins that are critical in the structure and function maintenance of the pulmonary alveolus by reducing surface tension at the air-liquid interface. The Immortalized Mouse Lung Epithelial Cells (MLE-15) is a cell line derived from transgenic FVB/N mice harbouring SV40 TAg under the control of the human SP-C promoter. Similar to alveolar type II cells, MLE-15 is characterized by the presence of apical microvilli and intracellular multiamellar inclusion bodies, as well as the ability to produce surfactant proteins B and C. This cell line is useful in studying the regulation of lung epithelial genes and elucidating the complex transcriptional networks related to airway/alveolar differentiation.
Source
Distal respiratory epithelium
Recommended Medium
SuperCult® Immortalized Mouse Lung Epithelial Cell Medium
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Markers
SP-A (low level), SP-B, SP-C
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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What research applications are Immortalized Mouse Lung Epithelial Cells (MLE-15) suitable for?
MLE-15 cells are highly versatile and can be utilized in numerous research areas, including:
Investigating lung development and differentiation processes.
Studying the pathophysiology of respiratory diseases, such as asthma and chronic obstructive pulmonary disease (COPD).
Researching the effects of environmental pollutants on lung cells.
Evaluating drug responses and therapeutic interventions in pulmonary medicine.
Exploring epithelial barrier function and homeostasis in lung tissue.
How should MLE-15 cells be cultured for optimal growth?
We provide a comprehensive culture protocol with each order that includes:
Recommended culture media and supplements necessary for optimal growth.
Guidelines for seeding densities and passaging techniques.
Required environmental conditions, including temperature, humidity, and CO2 levels.