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Immortalized Mouse Lung Epithelial Cells (MLE-12)

Cat.No.: CSC-I2116Z

Species: mouse

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I2116Z
Description
This line was established in 1992 by Kathryn A. Wikenheiser from pulmonary tumors in a mouse transgenic for the SV40 large T antigen under the control of the promoter region of the human surfactant protein C gene.The cells express the mRNA for large T antigen. Lung surfactant proteins B and C were detected. The cells secrete phospholipids in response to phorbol esters and ATP but not in response to forskolin.
Species
mouse
Recommended Medium
SuperCult® Immortalized Mouse Lung Epithelial Cell Medium (Cat No.: CM-I2116Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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