Description
Immortalized Mouse Hepatic Stellate Cells-SV40 were derived from WT mouse via enzymatic digestion and Percoll density gradient centrifugation. The primary cells were immortalized via transfection of the pCMV Simian Virus 40 Large T antigen. The Immortalized Mouse Hepatic Stellate Cells-SV40 are useful in studies focusing on liver fibrosis since it is the major cell type involved in the formation of scar tissue. The cells are particularly applicable in studies associated with inflammatory responses, as NF-κB and inflammatory cytokines levels have been found to change in the Immortalized Mouse Hepatic Stellate Cells upon LPS stimulation.
Recommended Medium
SuperCult® Immortalized Mouse Hepatic Stellate Cell Medium (Cat No.: CM-I9351L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Quality Control
1) Genotype analysis;
2) NF-κB Activation Assay;
3) Cytokine ELISA Assays;
4) 3[H]-thymidine Cell Proliferation Assay.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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What are the recommended culture conditions for Immortalized Mouse Hepatic Stellate Cells-SV40T?
We supply an optimal culture protocol, including recommended media composition, supplements, and environmental conditions required to sustain healthy growth and maintain stellate cell characteristics.