Immortalized Mouse Dendritic Cells (MutuDC1940)

Cat.No.: CSC-I9098L

Species: Mus musculus

Source: Spleen

Morphology: Small Aggregates

Culture Properties: Adherent

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Cat.No.

CSC-I9098L

Description

As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The Wild-type MutuDC1940 cell line is an immortalized splenic CD8α+subset (CD11chigh,B220-,DEC205+,CD24high,CD11b-) of conventional dendritic cells derived from the CD11c:SV40LgT transgenic mice.The MutuDC1940 cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Together with TLR3 knockout, TLR9 knockout and Ifnar1 knockout MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8α+subset.

Species

Mus musculus

Source

Spleen

Culture Properties

Adherent

Morphology

Small Aggregates

Immortalization Method

Isolated from C57BL/6 transgenic mice carrying SV40 Large T oncogene

Markers

CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4

Applications

For Research Use Only

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.

Shipping

Dry Ice.

Recommended Products

CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit

Quality Control

1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems;
2) proteome profile and surface markers assessed by RT-PCR; RT-PCR;
3) IL-12 cytokine secretion analyzed using ELISA;
4) Response to PAMP stimulation evaluated by functional assays.

BioSafety Level

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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