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Immortalized Mouse Dendritic Cells (DC2.4 )
Cat.No.: CSC-I2319Z
Species: Mouse
Culture Properties: Adherent and suspension
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Cat.No.
CSC-I2319Z
Description
DC2.4 are immortalized murine dendritic cells created by transducing bone marrow isolates of C57BL/6 mice with retrovirus vectors expressing murine granulocyte-macrophage CSF (GMCSF) and the myc and raf oncogenes. DC2.4 exhibits characteristic features of dendritic cells including cell morphology and the expression of dendritic cell-specific markers and the ability to phagocytose and present exogenous antigens on both MHC class I and class II molecules.
Species
Mouse
Recommended Medium
RPMI-1640, 10%FBS, 10ng/ml murineGM-CSF and 1% PS solution
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent and suspension
Immortalization Method
Murine granulocyte-macrophage CSF (GMCSF), the myc and raf oncogenes
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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