Description
Immortalized Mouse Corneal Epithelial Cells from Creative Bioarray were developed from mouse tissue transduced with a lentiviral expression vector containing the SV40T gene. Mouse Corneal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin, ZO-1. The cell line was continuously cultured for more than 50 population doublings without showing signs of growth retardation or replicative senescence.
Recommended Medium
SuperCult® Immortalized Mouse Corneal Epithelial Cell Medium (Cat No.: CM-I2070Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Quality Control
Real Time PCR was used to quantify SV40T and hTERT expression in immortalized cell line.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are Immortalized Mouse Corneal Epithelial Cells?
These are mouse-derived corneal epithelial cells that have been genetically modified to be cultured for more than 50 population doublings without showing signs of growth retardation or replicative senescence. They provide a reliable in vitro model for studying corneal epithelial biology and ocular surface research.
What research applications are Immortalized Mouse Corneal Epithelial Cells suitable for?
Immortalized Mouse Corneal Epithelial Cells (Cat No.: CSC-I2070Z) are ideal for a variety of applications, including ocular surface research, corneal development studies, wound healing investigations, drug testing, and toxicity assessments related to ophthalmic applications.
How do these cells compare to primary corneal epithelial cells?
Unlike primary cells, which have a limited lifespan and batch-to-batch variability, these immortalized cells offer a stable, reproducible model for long-term studies, ensuring consistent experimental results.