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Immortalized Mouse Cerebral Capillary Endothelial Cells (cEND)
Cat.No.: CSC-I9179L
Species: Mus musculus
Source: Cerebral cortex
Morphology: spindle shaped
Culture Properties: Adherent
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Note: Never can cells be kept at -20 °C.
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
2) Tight junction protein Claudin-5 assessed by immunostainning and claudin-1, -3 and -12 detected in mRNA level;
3) BBB marker protein Glut-1 and tight junction-associated protein occludin measured by western blot analysis.
The cell line's response to pro-inflammatory stimuli was measured by 1) TER, 2) western blot and 3) gene expression analysis using RT-PCR.
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Description: Follicle stimulating hormone (FSH) receptor, a receptor uniquely expressed in ovarian granulosa and testicular sertoli cells, play an important role in the regulation of oogenesis, spermatogenesis and steroid hormone production. Unfortunately FSH receptor expression is often lost within 1-2 days ofin vitroculture of primary cells. The Immortalized Rat Steroidogenic Granulosa Cells stably expressing FSH Receptor is responsive to FSH stimulation and produces progesterone as a result at levels comparable to primary cells. This cell line is useful in studying FSH-FSH receptor interactions, as well as how FSH receptor regulates the development and maturation of ovarian follicle. Together with theImmortalized Rat Steroidogenic Granulosa Cells expressing LH Receptor, researchers can compare the LH and FSH signaling in homologues cell systems.
Description: The Immortalized Mouse Retinal Pigmented Epithelial Cells- HPV E6/E7, derived from healthy C57B1/6 mouse RPE cells, is a cell line that retained many of theirin vivophenotypic characteristics. RPE-specific markers such as RPE65 and cellular retinaldehyde binding protein (CRALBP), as well as epithelial markers ZO1, cytokeratin 8 and 18 are expressed in the Immortalized Mouse Retinal Pigmented Epithelial Cells- HPV E6/E7. These cells are useful in studies of RPE cell biology and investigation of ocular disease including age related macular degeneration (AMD).
