Immortalized Human Cerebral Microvascular Endothelial Cells-SV40+ hTERT (HCMEC/D3)

Cat.No.: CSC-I2317Z

Species: homo sapiens

Morphology: Spindle-shaped, elongated

Culture Properties: Adherent

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Cat.No.

CSC-I2317Z

Description

Immortalized Human Cerebral Microvascular Endothelial Cells (HCMEC/D3) were derived from human temporal lobe microvessels isolated from tissue excised during surgery for control of epilepsy. The primary isolate was enriched in cerebral endothelial cells (CECs). In the first passage, cells were sequentially immortalized by lentiviral vector transduction with the catalytic subunit of human telomerase (hTERT) and SV40 large T antigen, following which CEC were selectively isolated by limited dilution cloning, and clones were extensively characterized for brain endothelial phenotype. This brain microvascular endothelial cell line represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS).

Species

homo sapiens

Tissue of Origin

Cerebral microvessel

Recommended Medium

SuperCult® Immortalized Human Cerebral Microvascular Endothelial Cell Medium (Cat No.: CM-I2317Z)

Freezing Medium

Complete medium supplemented with 10% (v/v) DMSO

Culture Properties

Adherent

Morphology

Spindle-shaped, elongated

Applications

hCMEC/D3 represents a stable, easily grown blood brain barrier (BBB) model cell line. It is ideal for drug uptake and active transport studies, as well as for understanding the brain endothelium response to various human pathogens and inflammatory stimuli.

For Research Use Only

Growth Properties

Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂.

Storage

Store in liquid nitrogen

Shipping

Ship in dry ice

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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