Description
Human Cardiomyocytes are isolated from human heart ventricles. Their specialized high-oxygen-content with a large number of mitochondria contributes to the major role of cardiac muscles in the heart’s rhythmic pumping. Cardiomyocytes are regulated by a complex network of signals. Cardiomyocytes hypertrophy and apoptosis have been associated with the loss of contractile function during heart failure. They are ideal models for study of cytokines and cellular signaling mechanisms that lead to myocyte death, as well as for research on mechanical strain and cell-cell interaction.
Immortalized Human Cardiomyocytes-SV40 were developed from human tissues transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 20 passages without showing signs of growth retardation or replicative senescence.
Recommended Medium
SuperCult® Immortalized Human Cardiomyocyte Medium (Cat No.: CM-I9071L)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Markers
sarcomeric alpha-actinin, slow muscle myosin
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Recommended Products
CSC-C2847 Human Cardiomyocytes
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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What research applications areImmortalized Human Cardiomyocytes-SV40 suitable for?
Immortalized Human Cardiomyocytes-SV40 are suitable for a variety of research applications, including:
Cardiac physiology and pathophysiology studies.
Investigating mechanisms of cardiac diseases and disorders.
Drug development and cardiotoxicity screening.
Research on ion channel function and cardiac electrophysiology.
Studies of cardiomyocyte response to mechanical or biochemical stimuli.