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Immortalized Human Brown Pre-adipocytes (PAZ6)

Cat.No.: CSC-I2365Z

Culture Properties: Adherent

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Cat.No.
CSC-I2365Z
Description
Immortalized human brown pre-adipocytes, derived from infant brown adipose tissue, were generated by microinjecting vascular stromal cells with the pUC 18 vector. This vector carries the SV40 large and small T antigen, regulated by a truncated human vimentin promoter, enabling cellular immortalization. These cells express both PPARγ and UCP1. Upon induction with a specific drug cocktail, they undergo terminal differentiation into brown adipocytes. These differentiated cells exhibit expression profiles and morphology comparable to primary brown adipocytes.
Recommended Medium
SuperCult® Immortalized Human Brown Pre-adipocyte Medium
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40T
Quality Control
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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