Description
Immortalized Human Bone Marrow-derived Mesenchymal Stromal Cells (hTERT, No viral gene) were developed from human tissues transduced by non-viral gene transfer of a plasmid carrying the hTERT gene. The cell line was continuously cultured for more than 50 population doublings without showing signs of growth retardation or replicative senescence. The cells have been verified to differentiate towards mesodermal lineages (adipocytes, chondrocytes, osteoblasts). This immortalized human mesenchymal stem cell lines have been established under conditions that will allow production of EVs.
Recommended Medium
SuperCult® Immortalized Human Bone Marrow-derived Mesenchymal Stromal Cell Medium (Cat No.: CM-I2107Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
Human telomerase reverse transcriptase (hTERT)
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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Can these cells be used for differentiation studies?
Yes, these cells retain multipotent capabilities, allowing differentiation into various lineages such as osteogenic, adipogenic, and chondrogenic pathways, making them ideal for studying differentiation and regenerative processes.
Are these cells suitable for cell-based therapy research?
Absolutely! Their consistent growth, multipotency, and non-viral modification make them a reliable model for exploring the potential of MSC-based therapeutic applications and regenerative medicine strategies.