Description
Immortalized Human Astrocytes-SV40T have been obtained immortalizing Human Cortical Astrocytes with SV40LT expressing lentiviral particles. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 5th passage while the SV40tranduced cells go beyond 30 passages.
Recommended Medium
SuperCult® Immortalized Human Astrocyte Medium (Cat No.: CM-C12025Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Quality Control
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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What are Immortalized Human Astrocytes-SV40T?
Immortalized Human Astrocytes (SV40T) are human astrocyte cells that have been genetically modified with the SV40 large T antigen to go beyond 30 passages. This allows them to be used continuously in research without the limitations associated with primary astrocyte cultures.
What research applications are Immortalized Human Astrocytes-SV40T suitable for?
These cells are ideal for various research applications, including neurobiology, neurodegenerative disease studies, toxicity testing, neuroinflammation research, and drug discovery targeting central nervous system (CNS) disorders.
Can these astrocytes be used in co-culture systems?
Absolutely. These astrocytes are suitable for co-culture with other cell types, such as neurons or microglia, to simulate the complex cellular environments of the CNS and study cell-cell interactions.
What characterization has been performed on these cells to show that they are astrocytes?