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Immortalized Bovine Skeletal Muscle Myoblasts

Cat.No.: CSC-I2302Z

Species: Bovine

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I2302Z
Description
Immortalized Bovine Skeletal Muscle Myoblasts have been obtained immortalizing Bovine Skeletal Muscle Myoblasts with SV40 gene. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 4th passage while the SV40 gene‐tranduced cells go beyond 20 passages. This cell line is a great in vitro model system.
Species
Bovine
Recommended Medium
SuperCult® Immortalized Bovine Skeletal Muscle Myoblast Cell Medium (Cat No.: CM-I2302Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Applications
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are Immortalized Bovine Skeletal Muscle Myoblasts?

Immortalized Bovine Skeletal Muscle Myoblasts (Cat No.: CSC-I2302Z) are genetically modified cells derived from bovine skeletal muscle. These cells have been engineered to go beyond 20 passages, overcoming the limitations of primary cells, and providing a stable and reliable model for muscle research.

Why should I use Immortalized Bovine Skeletal Muscle Myoblasts in my research?

Immortalized Bovine Skeletal Muscle Myoblasts (Cat No.: CSC-I2302Z) offer numerous advantages, such as consistent growth, repeated use, and the ability to provide high-quality, reproducible data. They are ideal for applications in muscle physiology, drug testing, gene function studies, and developmental biology.

What applications are suitable for Immortalized Bovine Skeletal Muscle Myoblasts?

Immortalized Bovine Skeletal Muscle Myoblasts (Cat No.: CSC-I2302Z) are perfect for a wide range of applications, including muscle development studies, disease modeling for muscle dystrophies, drug screening, and functional genomic studies focusing on muscle gene expression and regulation.

What are the benefits of using these myoblasts over primary bovine muscle cells?

Immortalized Bovine Skeletal Muscle Myoblasts (Cat No.: CSC-I2302Z) provide a uniform and indefinite cell source that reduces variability and enhances reproducibility in experiments. Unlike primary cells, which have a limited lifespan and can show donor variability, immortalized cells offer a more reliable alternative for long-term studies.

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For research use only. Not for any other purpose.