BA/F3

Cat.No.: CSC-C2045

Species: Mouse

Source: pro B cells

Morphology: round (some polymorph) cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C2045

Description

IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references.

Species

Mouse

Source

pro B cells

Recommended Medium

90%RPMI-1640 + 10% FBS + 10 ng/ml Recombinant Mouse IL-3

Culture Properties

suspension

Morphology

round (some polymorph) cells in suspension

Karyotype

Murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n>

Reference

Publication

Applications

a. Kinase drug discovery. Ba/F3, a murine interleukin-3 dependent pro-B cell line is increasingly popular as a model system for assessing both the potency and downstream signaling of kinase oncogenes, and the ability of small-molecule kinase inhibitors to block kinase activity.
b. Cell-Based assay
c. In vivo efficacy study

Quality Control

Mycoplasma: negative in PCR assays
Immunology: CD11b (+), F4/80 (+)
Viruses: PCR: SMRV -

Storage and Shipping

Ship in dry ice.
Store in liquid nitrogen.

BioSafety Level

BSL–1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Can you provide the QC of the cell line? For example, mycoplasma test? Do you have the genomic data to make sure there's no contamination.

Yes, of course. We can provide you with the COA of BA/F3, which includes QC test results of each lot.

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