WILL-1

Cat.No.: CSC-C0633

Species: Human

Source: B cell lymphoma

Morphology: small round or teardrop-shaped cells growing singly or in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0633

Description

Established in 2007 from the bone marrow mononuclear cells of an 82-year-old Japanese man with diffuse large B-cell lymphoma in the leukemic phase

Species

Human

Source

B cell lymphoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

small round or teardrop-shaped cells growing singly or in clumps in suspension

Karyotype

Human bimodal hypodiploid/hyperdiploid karyotype; 45(41-46)<2n>X, -Y, add(1)(q24), der(2)t(1;2)(q12;q3?7)inv(1)(q2?4q4?1), i(4q), der(6)t(6;8)(q27;q24), t(8;14)(q24;q32), der(17)t(X;17)(q23;p13), i(21q); sdl with idem, 57(50-58)<2n>X, -Y, +5, +6, +7, +8,

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +,CD80 +, CD138 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the difference between DMEM and RPMI media?

RPMI is a media used widely to culture mammalian cells in suspension culture. DMEM is a modified type of basal medium, with increased amino acid and vitamin concentrations up to fourfold. DMEM is used in culturing cells in adherent cultures.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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Creative Bioarray provides detailed documentation and quality assurance certificates for all tumor cell products, ensuring full traceability and accountability.

09 Jan 2022

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