WEHI-164S

Cat.No.: CSC-C1057

Species: Mouse

Source: fibrosarcoma

Morphology: fibroblast-like cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C1057

Description

Established from a fibrosarcoma chemically induced in a BALB/c mouse; cells were described to be sensitive to human cytotoxic monocytes, to tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta)

Species

Mouse

Source

fibrosarcoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

fibroblast-like cells growing as monolayers

Karyotype

Murine hypertriploid karyotype with 20% polyploidy - 60-62<3n> - supernumerary ring/acf but no centric fusion marker present in most cells

Quality Control

Mycoplasma: negative in DAPI, microbiological culture assays
Immunology: see available graph: lysis of MTT-labelled cells exposed to TNF
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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