UT-7

Cat.No.: CSC-C0294

Species: Human

Source: Bone marrow

Morphology: round (relatively large) cells growing singly in suspension

Culture Properties: Suspension

Inquire for Price

Specification
Q & A
Customer Review
Publications

Cat.No.

CSC-C0294

Description

established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7); cells are constitutively cytokine-dependent and responsive to various cytokines

Species

Human

Source

Bone marrow

Recommended Medium

RPMI 1640 w/o Hepes + 10% FBS +5-7u/ml Epo

Culture Properties

Suspension

Morphology

round (relatively large) cells growing singly in suspension

STR DNA Profile

Amelogenin: X,Y
D5S818: 12
D13S317: 8
D7S820: 8
D16S539: 10,12
vWA: 14,18
TH01: 6,9
TPOX: 10
CSF1PO: 12
D19S433: 14,14.2
D21S11: 31.2
D18S51: 14,17
D6S1043: 10,19
D3S1358: 16
Penta D: 9,11
D2S441: 10,11
D8S1179: 11,15
Penta E: 16,17
D12S391: 18,19
D2S1338: 18,23
FGA: 23

Karyotype

human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7q)x1-2, add(8)(q24)x1-3, del(9)(q22), add(12)(p12-13), der(13)t(13;13)(p11;q14), add(14)(p11), add(15)(p11), add(16)(p1?), add(17)(p11), add(19)(p13)x2, add(19)(q13), i(21q)x1-2 - extensive subclonal variation present - resembles published karyotype

Reference

Publication

Quality Control

Mycoplasma: negative in PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, CD41 (+), CD42 (+), CD68 +, CD235a (+), HLA-DR (+)
Viruses: ELISA: reverse transcriptase negative

Storage and Shipping

ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why wash cell culture with PBS?

PBS is often used for washing due to being isotonic and non-toxic to cells and tissues and thus allows for cells to be rinsed of unwanted media without potentially lysing them.

Ask a Question