U-HO1

Cat.No.: CSC-C6217X

Species: Human

Source: Hodgkin lymphoma

Morphology: polymorphic cells growing singly and in clumps in suspension; occasionally large cells present

Culture Properties: suspension

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Cat.No.

CSC-C6217X

Description

Established in 2005 from the pleural effusion of a 23-year-old man with terminal refractory classical Hodgkin lymphoma (nodular sclerosing type) after radiotherapy, salvage chemotherapy and autologous stem cell transplantation; described to carry an amplification of REL-BCL11A region with overexpression of miR-216/217

Species

Human

Source

Hodgkin lymphoma

Recommended Medium

80% SuperCult® IMDM + RPMI-1640 (at 4:1) + 20% h.i. FBS, 2 mM L-glutamine

Culture Properties

suspension

Morphology

polymorphic cells growing singly and in clumps in suspension; occasionally large cells present

Karyotype

Human hyperdiploid karyotype with 9% polyploidy; 51(46-52)<2n>XY, +5, +6, +8, +12, +17, +20, -22, +mar, del(1)(p13p31), der(2)qdp(2)(p1?5p1?6)t(2;10)(p24;q25.2), add(4)(q35), t(4;6)(p15.2;p21), del(5)(q11q34), der(5)t(5;15)(p11;q13), t(5;22)(q35;q13), del

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 -, CD30 +, CD34 -, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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What is vitro cell culture experiments?

In vitro cell culture is a method used for studying the behavior of animal cells in a controlled environment, free of systemic variations. Currently, different types of cell cultures have been adapted and developed.

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