U-2940

Cat.No.: CSC-C0625

Species: Human

Source: B cell lymphoma

Morphology: small round cells growing singly and in clusters in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0625

Description

Established in 1991 from the pleural effusion of a 18-year-old woman with diffuse large B-cell lymphoma (DLBCL) after being diagnosed earlier with Hodgkin lymphoma and treated with chemotherapy; cells were described to bear microsatellite instability

Species

Human

Source

B cell lymphoma

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

small round cells growing singly and in clusters in suspension

Karyotype

Human hypodiploid karyotype with 8% polyploidy; 45(43-45)X, -X, del(3)(p14p21), del(6)(q13q15), der(7)t(2;7)(q22;p22), dup(12)(q13q22), der(14)t(X;14)(q12;p11), t(16;16)(p12;p13), del(17)(p13); carries homozygous microdeletion of SOCS1 locus; resembles pu

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, cm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the STR profiling method?

Genotyping of STR loci is performed by converting the size of amplicons obtained to the number of core sequence repeats using an allelic ladder. The allelic ladder is a marker specifically developed for STR analysis. For each locus analyzed, the number of STR copies for each allele is determined.

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