TMM

Cat.No.: CSC-C0246

Species: Human

Source: B lymphoblastoid cells (from patient with chronic myeloid leukemia in blast crisis)

Morphology: polygonal cells, single or in clusters in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0246

Description

Established from the peripheral blood of a 62-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) in blast crisis in 1985; cells were described to be Ph-negative, BCR-ABL1-negative; cells appear to be rather a "normal" B-lymphoblastoid cell line (B-LCL)

Species

Human

Source

B lymphoblastoid cells (from patient with chronic myeloid leukemia in blast crisis)

Recommended Medium

90%SuperCult® IMDM + 10% h.i. FBS

Culture Properties

suspension

Morphology

polygonal cells, single or in clusters in suspension

Karyotype

Human near-diploid karyotype with 8% polyploidy - 46(42-46)<2n>XY - small ring present in 6% cells - no consistent numerical or structural rearrangement - resembles published karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture assays
Immunology: CD3 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD33 -, CD37 +, HLA-DR +, anti-EBNA2 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -
Viruses: ELISA: reverse transcripta

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5-10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Why does media turn yellow?

A yellow hue to the medium indicates the pH is too low (acidic), a purple hue shows the pH is too high (alkaline) whereas an orangey-red hue indicates the medium is of the correct physiological pH.

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