TM4

Cat.No.: CSC-C9739L

Species: Mouse

Source: testis; Sertoli cell

Morphology: epithelial

Culture Properties: monolayer

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Cat.No.

CSC-C9739L

Description

Species: mouse - male, 11 to 13 days old
Strain: BALB/c nu/+
Receptor: follicle stimulating hormone (FSH); androgen; estrogen; progesterone
Histocompatibility: H-Y
Production: transferrin; H-Y antigen; retinol binding protein; plasminogen activator
Histopathology: normal
Note: the TM4 cell line responds to FSH with an increase in cAMP production, but does not respond to luteinizing hormone (LH); the FSH responsiveness is much reduced compared to primary Sertoli cell cultures; constitutive plasminogen activator production is low, but is stimulated by FSH and, to a greater extent, by retinoic acid; tested and found negative for ectromelia virus (mousepox)

Species

Mouse

Source

testis; Sertoli cell

Recommended Medium

DMEM/Ham's F12, 92.5%; horse serum, 5%; h.i. FBS, 2.5%

Culture Properties

monolayer

Morphology

epithelial

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

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