TM3

Cat.No.: CSC-C9738L

Species: Mouse

Source: testis; Leydig cell

Morphology: epithelial

Culture Properties: monolayer

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Cat.No.

CSC-C9738L

Description

Species: mouse - male, 11 to 13 days old
Strain: BALB/c nu/+
Receptor: luteinizing hormone (LH); epidermal growth factor(EGF); androgen; estrogen; progesterone
Production: prostaglandin F2a
Histopathology: normal
Note: the TM3 cell line responds to LH with an increase in cAMP production, but does not respond to follicle stimulating hormone (FSH); the maintenance of responsiveness to LH is dependent upon serum lot; in the presence of LH, the cells are capable of metabolizing cholesterol; tested and found negative for ectromelia virus (mousepox)

Species

Mouse

Source

testis; Leydig cell

Recommended Medium

DMEM/Ham's F12, 92.5%; horse serum, 5%; h.i. FBS, 2.5%

Culture Properties

monolayer

Morphology

epithelial

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

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