TK-6

Cat.No.: CSC-C0698

Species: Homo sapiens, human

Source: lymphoblast

Morphology: round cells growing singly in suspension

Culture Properties: Suspension

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0698

Description

established 1995 from the pleural effusion of a 30-year-old man with chronic myelogenous leukemia (CML) in T cell lineage blast crisis after bone marrow transplantation; cells carry the Philadelphia chromosome with a e14-a2 (b3-a2) BCR-ABL1 fusion gene. TK6 cells are CD19 positive, 50% of the cell population express CD20 and a small sub-population has been tested positive for CD22. This cell line has been shown to be negative for Bovine Viral Diarrhoea Virus (BVDV).

Species

Homo sapiens, human

Source

lymphoblast

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

Suspension

Morphology

round cells growing singly in suspension

STR DNA Profile

Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20

Karyotype

human hyperdiploid karyotype; 48(46-50)<2n>XY, +22, +22, del(1)(q24), del(6)(q21q24), dup(7)(p1?4q3?5)inv(7)(p14q35), t(9;22)(q34;q11), add(21)(p11), der(22)?inv(22)(p11q11)t(9;22)(q34;q11)x2; carries complex t(9;22) effecting BCR-ABL1 rearrangement with 3x genomic amplification in der(22); matches published karyotype

Applications

This cell line can be used in bromodeoxyuridine resistant assays.

Disease

chronic myeloid leukemia

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

liquid nitrogen vapor phase

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is used to stain cell membranes?

Typically, dyes of a lipophilic nature are used; however, they internalize rapidly, offering a very narrow window for imaging. Fluorescently labeled lectins, such as wheat germ agglutinin, have also been employed as plasma membrane stains.

Ask a Question