TI-4

Cat.No.: CSC-C6181X

Species: Mouse

Source: hybridoma (anti-rat fiber/troponin I)

Morphology: cells grow in suspension as single cells or in small clumps

Culture Properties: suspension

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Cat.No.

CSC-C6181X

Description

Described as secreting a mouse monoclonal antibody (IgG1) reacting with rat cardiac fibers in immunofluorescence and with rat adult and fetal cardiac troponin I in Western blotting; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified troponin complex from bovine adult heart

Species

Mouse

Source

hybridoma (anti-rat fiber/troponin I)

Recommended Medium

80% DMEM ) + 10-20% horse serum.

Culture Properties

suspension

Morphology

cells grow in suspension as single cells or in small clumps

Karyotype

murine karyotype

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% horse serum, 10% DMSO at about 4-6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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