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SUP-M2

Cat.No.: CSC-C0518

Species: Human

Source: anaplastic large cell lymphoma

Morphology: round cells growing singly or in clumps in suspension

Culture Properties: suspension

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Cat.No.
CSC-C0518
Description
Derived from the cerebrospinal fluid of a 5-year-old girl with refractory malignant histiocytosis; described to carry the typical t(2;5)(p23;q35) leading to the NPM1-ALK (NPM-ALK) fusion gene and to express CD30, defining the cells as anaplastic large cell lymphoma (ALCL)
Species
Human
Source
anaplastic large cell lymphoma
Recommended Medium
Culture Properties
suspension
Morphology
round cells growing singly or in clumps in suspension
Karyotype
Human flat-moded near-diploid karyotype with 3.5% polyploidy - 44-50<2n>XX/XXX, +1, +7, +8, add(X)(q26), der(X;7)(q10;p10), add(1)(q21), der(1)(1pter->q43::1q43->q21::9q31->qter), t(2;5)(p23;q35), der(6)t(6;6)(q27;p11), add(9)(q31), add(11)(q24)/der(11)t(
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 +, CD3 -, CD4 +, CD13 -, CD14 -, CD19 -, CD21 -, CD25 +, CD30 +, CD34 -, CD71 +, HLA-DR +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 7.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

SUP-M2 cells are a human cell line derived from the cerebrospinal fluid of a 5-year-old girl with refractory malignant histiocytosis. These cells are characterized by specific genetic and phenotypic features, making them an important model for studying a particular type of cancer known as anaplastic large cell lymphoma (ALCL).

The SUP-M2 cell line is notable for carrying the characteristic translocation t (2; 5) (p23; q35). This translocation leads to the fusion of two genes, NPM1 and ALK, resulting in the formation of the NPM1-ALK fusion gene. The NPM1-ALK fusion gene is a hallmark genetic alteration in a subset of ALCL cases and plays a crucial role in the development and progression of this disease.

In addition to the genetic abnormality, SUP-M2 cells also express the CD30 antigen. CD30 is a cell surface marker that is commonly observed on ALCL cells. The presence of CD30 further defines SUP-M2 cells as anaplastic large-cell lymphoma and contributes to their phenotypic characteristics.

The SUP-M2 cell line has been widely used in research to investigate the biological and molecular aspects of ALCL, including the role of the NPM1-ALK fusion gene, cellular signaling pathways, therapeutic targets, and drug testing. By studying SUP-M2 cells, researchers aim to gain insights into the pathogenesis of ALCL and develop more effective treatment strategies for this type of lymphoma.

B7-H3 Redirected CAR-T Cells Control ALCLs In Vitro

B7 homolog 3 (B7-H3), also named CD276, is a member of the B7 family of immune regulatory proteins. B7-H3 CAR-T cells showed strong cytotoxicity and cytokine secretion against target ALCL cells (SUP-M2, SU-DHL-1, and Karpas 299) in vitro. Cytotoxicity was measured using two different approaches LDH release cytotoxic assay and luciferase-based assay in various E: T ratios. Increased cytotoxicity along with an increased E: T ratio was observed in B7-H3 CAR-T cells to target ALCL cell lines in both approaches. Furthermore, >60% specific lysis was reached when the E: T ratio was 5:1 for all targets in the LDH release cytotoxic assay (Fig. 1A).

To confirm this effect, stable expressing luciferase cell lines were generated from parental SUP-M2, Karpas299, and SU-DHL-1 cell lines as well as the performed luciferase-based assay. This was consistent with data from the LDH release cytotoxic assay, and B7-H3 CAR-T cells showed their potency in controlling SUP-M2, Karpas299, and SU-DHL-1 (Fig. 1B). The secreting of IL-2 and IFNγ was observed at a high level when B7-H3 T cells were co-cultured with ALCLs. In contrast, UTD and CD19 CAR-T control cells showed minimal secretion of both cytokines (Fig. 1C and D).

B7-H3 T cell effects on B7-H3-expressing ALCL cells (SUP-M2, SU-DHL-1, and Karpas 299).Fig. 1 B7-H3 T cell effects on B7-H3-expressing ALCL cells (SUP-M2, SU-DHL-1, and Karpas 299). (Slupianek A and Skorski T, 2004)

What is tumor monoclonality?

According to educational materials provided by the National Institutes of Health, monoclonal tumor origin means that tumors are derived from a single ancestral cell that underwent conversion from a normal to a cancerous state.

What is the significance of the t(2;5)(p23;q35) translocation in SUP-M2 cells?

The t(2;5)(p23;q35) translocation in SUP-M2 cells leads to the formation of the NPM1-ALK fusion gene. This genetic alteration is characteristic of a subset of ALCL cases and is crucial in the development and progression of this type of lymphoma.

What is the role of CD30 in SUP-M2 cells?

CD30 is a cell surface marker expressed by SUP-M2 cells. It is commonly observed on ALCL cells and aids in defining SUP-M2 cells as anaplastic large cell lymphoma. CD30 is involved in various signaling pathways and is a target for therapeutic interventions in ALCL.

Are there any other research applications for SUP-M2 cells?

Yes, apart from ALCL-related research, SUP-M2 cells can be utilized to investigate broader aspects of cancer biology, including cellular signaling pathways, genetic alterations, and drug response mechanisms. They provide a valuable resource for studying malignant histiocytosis and other relevant diseases.

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Average Rating: 4.7    |    3 Scientist has reviewed this product

Excellent results

The tumor cells are easy to culture and have provided excellent results in our experiments.

03 Nov 2022


Ease of use

After sales services

Value for money


Satisfied

I recently purchased SUP-M2 cell products from Creative Bioarray, and I am extremely satisfied with the quality and performance of their cells.

29 Jan 2024


Ease of use

After sales services

Value for money


In excellent condition

The SUP-M2 cells were delivered in excellent condition, exhibiting the expected phenotypic characteristics of ALCL. The documentation provided, including certificates of analysis and handling instructions, was comprehensive and helpful for my research.

23 Aug 2023


Ease of use

After sales services

Value for money


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For research use only. Not for any other purpose.